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Quantitative spectroscopy of single molecule interaction times

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Item Type:Article
Title:Quantitative spectroscopy of single molecule interaction times
Creators Name:Boltz, H.H. and Sirbu, A. and Stelzer, N. and Lohse, M.J. and Schütte, C. and Annibale, P.
Abstract:Single molecule fluorescence tracking provides information at nanometer-scale and millisecond-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address this aspect by developing a theoretical foundation for a spectroscopy of interaction times, i.e., the inference of interaction from imaging data. A non-trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted, together with the dependence of the exponential term upon the microscopic reaction affinity. Our approach is validated with simulated and experimental datasets.
Keywords:Optics, Optoelectronic Devices, Biological Environments, Dynamic Behaviors, Exponential Behaviors, Isolated Molecules, Quantitative Spectroscopy, Single Molecule Fluorescence, Temporal Resolution, Theoretical Foundations, Molecules
Source:Optics Letters
Publisher:Optical Society of America
Page Range:1538-1541
Date:1 April 2021
Additional Information:Copyright © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved. - This supplement published with The Optical Society on 19 March 2021 by The Authors under the terms of the Creative Commons Attribution 4.0 License in the format provided by the authors and unedited. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Official Publication:https://doi.org/10.1364/OL.413030
PubMed:View item in PubMed

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