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Quantitative spectroscopy of single molecule interaction times

Item Type:Article
Title:Quantitative spectroscopy of single molecule interaction times
Creators Name:Boltz, H.H. and Sirbu, A. and Stelzer, N. and Lohse, M.J. and Schütte, C. and Annibale, P.
Abstract:Single molecule fluorescence tracking provides information at nanometer-scale and millisecond-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address this aspect by developing a theoretical foundation for a spectroscopy of interaction times, i.e., the inference of interaction from imaging data. A non-trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted, together with the dependence of the exponential term upon the microscopic reaction affinity. Our approach is validated with simulated and experimental datasets.
Keywords:Optics, Optoelectronic Devices, Biological Environments, Dynamic Behaviors, Exponential Behaviors, Isolated Molecules, Quantitative Spectroscopy, Single Molecule Fluorescence, Temporal Resolution, Theoretical Foundations, Molecules
Source:Optics Letters
ISSN:0146-9592
Publisher:Optical Society of America
Volume:46
Number:7
Page Range:1538-1541
Date:1 April 2021
Official Publication:https://doi.org/10.1364/OL.413030
PubMed:View item in PubMed

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