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| Item Type: | Article | ||||
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| Title: | Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror | ||||
| Creators Name: | Vladimirov, N. and Preusser, F. and Wisniewski, J. and Yaniv, Z. and Desai, R.A. and Woehler, A. and Preibisch, S. | ||||
| Abstract: | Light-sheet microscopy has become indispensable for imaging developing organisms, and imaging from multiple directions (views) is essential to improve its spatial resolution. We combine multi-view light-sheet microscopy with microfluidics using adaptive optics (deformable mirror) which corrects aberrations introduced by the 45°-tilted glass coverslip. The optimal shape of the deformable mirror is computed by an iterative algorithm that optimizes the point-spread function in two orthogonal views. Simultaneous correction in two optical arms is achieved via a knife-edge mirror that splits the excitation path and combines the detection paths. Our design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experiments and high-content screening. | ||||
| Keywords: | Animals, Caenorhabditis elegans | ||||
| Source: | Biomedical Optics Express | ||||
| ISSN: | 2156-7085 | ||||
| Publisher: | Optical Society of America | ||||
| Volume: | 12 | ||||
| Number: | 4 | ||||
| Page Range: | 2186-2203 | ||||
| Date: | 1 April 2021 | ||||
| Official Publication: | https://doi.org/10.1364/BOE.416737 | ||||
| PubMed: | View item in PubMed | ||||
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