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Vasohibin-1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk

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Item Type:Article
Title:Vasohibin-1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk
Creators Name:Bastos de Oliveira, M. and Meier, K. and Jung, S. and Bartels-Klein, E. and Coxam, B. and Geudens, I. and Szymborska, A. and Skoczylas, R. and Fechner, I. and Koltowska, K. and Gerhardt, H.
Abstract:Previous studies have shown that Vasohibin-1 (Vash1) is stimulated by VEGFs in endothelial cells and that its overexpression interferes with angiogenesis in vivo. Recently, Vash1 was found to mediate tubulin detyrosination, a post-translational modification that is implicated in many cell functions, such as cell division. Here we used the zebrafish embryo to investigate the cellular and subcellular mechanisms of Vash1 on endothelial microtubules during formation of the trunk vasculature. We show that microtubules within venous-derived secondary sprouts are strongly and selectively detyrosinated in comparison with other endothelial cells, and that this difference is lost upon vash1 knockdown. Vash1 depletion in zebrafish specifically affected secondary sprouting from the posterior cardinal vein, increasing endothelial cell divisions and cell number in the sprouts. We show that altering secondary sprout numbers and structure upon Vash1 depletion leads to defective lymphatic vessel formation and ectopic lymphatic progenitor specification in the zebrafish trunk.
Keywords:Lymphangiogenesis, Vasohibin-1, Microtubules, Tubulin Detyrosination, Sprouting Angiogenesis, Animals, Zebrafish
Source:Development
ISSN:0950-1991
Publisher:Company of Biologists
Volume:148
Number:4
Page Range:dev194993
Date:19 February 2021
Official Publication:https://doi.org/10.1242/dev.194993
PubMed:View item in PubMed
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https://edoc.mdc-berlin.de/18931/Preprint version

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