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SIGLEC1 (CD169): a marker of active neuroinflammation in the brain but not in the blood of MS patients

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Title:SIGLEC1 (CD169): a marker of active neuroinflammation in the brain but not in the blood of MS patients
Creators Name:Ostendorf, L. and Dittert, P. and Biesen, R. and Duchow, A. and Stiglbauer, V. and Ruprecht, K. and Seelow, D. and Niesner, R.A. and Hauser, A.E. and Paul, F. and Radbruch, H.
Abstract:OBJECTIVE: We aimed to evaluate SIGLEC1 (CD169) as a biomarker in Multiple Sclerosis (MS) and Neuromyelitis optica spectrum disorder (NMOSD) and to evaluate the specificity of SIGLEC1+ myeloid cells for demyelinating diseases. METHODS: We performed flow cytometry-based measurements of SIGLEC1 expression on monocytes in 86 MS patients, 41 NMOSD patients and 31 healthy controls. Additionally, we histologically evaluated the presence of SIGLEC1+ myeloid cells in acute and chronic MS brain lesions as well as other neurological diseases. RESULTS: We found elevated SIGLEC1 expression in 16/86 (18.6%) MS patients and 4/41 (9.8%) NMOSD patients. Almost all MS patients with high SIGLEC1 levels received exogenous interferon beta as an immunomodulatory treatment and only a small fraction of MS patients without interferon treatment had increased SIGLEC1 expression. SIGLEC1+ myeloid cells were abundantly present in active MS lesions as well as in a range of acute infectious and malignant diseases of the central nervous system, but not chronic MS lesions. CONCLUSION: In our cohort, SIGLEC1 expression on monocytes was – apart from those patients receiving interferon treatment – not significantly increased in patients with MS and NMOSD, nor were levels associated with more severe disease. The presence of SIGLEC1+ myeloid cells in brain lesions could be used to investigate the activity in an inflammatory CNS lesion.
Publisher:Cold Spring Harbor Laboratory Press
Article Number:2020.12.18.20248500
Date:22 December 2020
Official Publication:https://doi.org/10.1101/2020.12.18.20248500

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