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Regulatory encoding of quantitative variation in spatial activity of a Drosophila enhancer

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Item Type:Article
Title:Regulatory encoding of quantitative variation in spatial activity of a Drosophila enhancer
Creators Name:Le Poul, Y. and Xin, Y. and Ling, L. and Mühling, B. and Jaenichen, R. and Hörl, D. and Bunk, D. and Harz, H. and Leonhardt, H. and Wang, Y. and Osipova, E. and Museridze, M. and Dharmadhikari, D. and Murphy, E. and Rohs, R. and Preibisch, S. and Prud'homme, B. and Gompel, N.
Abstract:Developmental enhancers control the expression of genes prefiguring morphological patterns. The activity of an enhancer varies among cells of a tissue, but collectively, expression levels in individual cells constitute a spatial pattern of gene expression. How the spatial and quantitative regulatory information is encoded in an enhancer sequence is elusive. To link spatial pattern and activity levels of an enhancer, we used systematic mutations of the yellow spot enhancer, active in developing Drosophila wings, and tested their effect in a reporter assay. Moreover, we developed an analytic framework based on the comprehensive quantification of spatial reporter activity. We show that the quantitative enhancer activity results from densely packed regulatory information along the sequence, and that a complex interplay between activators and multiple tiers of repressors carves the spatial pattern. Our results shed light on how an enhancer reads and integrates trans-regulatory landscape information to encode a spatial quantitative pattern.
Keywords:Drosophila Proteins, Genetic Enhancer Elements, Developmental Gene Expression Regulation, Transcription Factors, Animal Wings / Metabolism, Animals, Drosophila
Source:Science Advances
Publisher:American Association for the Advancement of Science
Page Range:eabe2955
Date:2 December 2020
Official Publication:https://doi.org/10.1126/sciadv.abe2955
PubMed:View item in PubMed

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