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Induced pluripotent stem cells of patients with Tetralogy of Fallot reveal transcriptional alterations in cardiomyocyte differentiation

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Item Type:Article
Title:Induced pluripotent stem cells of patients with Tetralogy of Fallot reveal transcriptional alterations in cardiomyocyte differentiation
Creators Name:Grunert, M. and Appelt, S. and Schönhals, S. and Mika, K. and Cui, H. and Cooper, A. and Cyganek, L. and Guan, K. and Sperling, S.R.
Abstract:Patient-specific induced pluripotent stem cells (ps-iPSCs) and their differentiated cell types are a powerful model system to gain insight into mechanisms driving early developmental and disease-associated regulatory networks. In this study, we use ps-iPSCs to gain insights into Tetralogy of Fallot (TOF), which represents the most common cyanotic heart defect in humans. iPSCs were generated and further differentiated to cardiomyocytes (CMs) using standard methods from two well-characterized TOF patients and their healthy relatives serving as controls. Patient-specific expression patterns and genetic variability were investigated using whole genome and transcriptome sequencing data. We first studied the clonal mutational burden of the derived iPSCs. In two out of three iPSC lines of patient TOF-01, we found a somatic mutation in the DNA-binding domain of tumor suppressor P53, which was not observed in the genomic DNA from blood. Further characterization of this mutation showed its functional impact. For patient TOF-02, potential disease-relevant differential gene expression between and across cardiac differentiation was shown. Here, clear differences at the later stages of differentiation could be observed between CMs of the patient and its controls. Overall, this study provides first insights into the complex molecular mechanisms underlying iPSC-derived cardiomyocyte differentiation and its transcriptional alterations in TOF.
Keywords:Cardiac Myocytes, Case-Control Studies, Cell Differentiation, Clone Cells, DNA Sequence Analysis, Developmental Gene Expression Regulation, Genetic Association Studies, Genetic Transcription, Germ-Line Mutation, Induced Pluripotent Stem Cells, Mutation, Single Nucleotide Polymorphism, Skin, Tetralogy of Fallot
Source:Scientific Reports
Publisher:Nature Publishing Group
Page Range:10921
Date:2 July 2020
Official Publication:https://doi.org/10.1038/s41598-020-67872-z
PubMed:View item in PubMed

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