Single cell tri-channel-processing reveals structural variation landscapes and complex rearrangement processes
| Item Type: | Preprint |
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| Title: | Single cell tri-channel-processing reveals structural variation landscapes and complex rearrangement processes |
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| Creators Name: | Sanders, A.D. and Meiers, S. and Ghareghani, M. and Porubsky, D. and Jeong, H. and van Vliet, M.A.C.C. and Rausch, T. and Richter-Pechańska, P. and Kunz, J.B. and Jenni, S. and Raeder, B. and Kinanen, V. and Zimmermann, J. and Benes, V. and Schrappe, M. and Mardin, B.R. and Kulozik, A. and Bornhauser, B. and Bourquin, J.P. and Marschall, T. and Korbel, J.O. |
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| Abstract: | Structural variation (SV), where rearrangements delete, duplicate, invert or translocate DNA segments, is a major source of somatic cell variation. It can arise in rapid bursts, mediate genetic heterogenity, and dysregulate cancer-related pathways. The challenge to systematically discover SVs in single cells remains unsolved, with copy-neutral and complex variants typically escaping detection. We developed single cell tri-channel-processing (scTRIP), a computational framework that jointly integrates read depth, template strand and haplotype phase to comprehensively discover SVs in single cells. We surveyed SV landscapes of 565 single cell genomes, including transformed epithelial cells and patient-derived leukemic samples, and discovered abundant SV classes including inversions, translocations and large-scale genomic rearrangements mediating oncogenic dysregulation. We dissected the ‘molecular karyotype’ of the leukemic samples and examined their clonal structure. Different from prior methods, scTRIP also enabled direct detection and discrimination of SV mutational processes in individual cells, including breakage-fusion-bridge cycles. scTRIP will facilitate studies of clonal evolution, genetic mosaicism and somatic SV formation, and could improve disease classification for precision medicine. |
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| Source: | bioRxiv |
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| Title of Book: | Single cell tri-channel-processing reveals structural variation landscapes and complex rearrangement processes |
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| Publisher: | Cold Spring Harbor Laboratory Press |
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| Article Number: | 849604 |
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| Date: | 21 November 2019 |
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| Official Publication: | https://doi.org/10.1101/849604 |
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