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breakpointR: an R/Bioconductor package to localize strand state changes in Strand-seq data

Item Type:Article
Title:breakpointR: an R/Bioconductor package to localize strand state changes in Strand-seq data
Creators Name:Porubsky, D. and Sanders, A.D. and Taudt, A. and Colomé-Tatché, M. and Lansdorp, P.M. and Guryev, V.
Abstract:MOTIVATION: Strand-seq is a specialized single-cell DNA sequencing technique centered around the directionality of single-stranded DNA. Computational tools for Strand-seq analyses must capture the strand-specific information embedded in these data. RESULTS: Here we introduce breakpointR, an R/Bioconductor package specifically tailored to process and interpret single-cell strand-specific sequencing data obtained from Strand-seq. We developed breakpointR to detect local changes in strand directionality of aligned Strand-seq data, to enable fine-mapping of sister chromatid exchanges, germline inversion and to support global haplotype assembly. Given the broad spectrum of Strand-seq applications we expect breakpointR to be an important addition to currently available tools and extend the accessibility of this novel sequencing technique. AVAILABILITY AND IMPLEMENTATION: R/Bioconductor package https://bioconductor.org/packages/breakpointR.
Keywords:DNA Sequence Analysis, Software
Publisher:Oxford University Press
Page Range:1260-1261
Date:15 February 2020
Official Publication:https://doi.org/10.1093/bioinformatics/btz681
PubMed:View item in PubMed

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