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Olig3 acts as a master regulator of cerebellar development

Item Type:Preprint
Title:Olig3 acts as a master regulator of cerebellar development
Creators Name:Lowenstein, E.D. and Rusanova, A. and Stelzer, J. and Hernaiz-Llorens, M. and Schroer, A.E. and Epifanova, E. and Bladt, F. and Isik, E.G. and Jia, S. and Tarabykin, V. and Hernandez-Miranda, L.R.
Abstract:The mature cerebellum controls motor skill precision and participates in other sophisticated brain functions that include learning, cognition and speech. Different types of GABAergic and glutamatergic cerebellar neurons originate in temporal order from two progenitor niches, the ventricular zone and rhombic lip, which express the transcription factors Ptf1a and Atoh1, respectively. However, the molecular machinery required to specify the distinct neuronal types emanating from these progenitor zones is still unclear. Here, we uncover the transcription factor Olig3 as a major determinant in generating the earliest neuronal derivatives emanating from both progenitor zones. In the rhombic lip, Olig3 regulates progenitor cell proliferation. In the ventricular zone, Olig3 safeguards Purkinje cell specification by curtailing the expression of Pax2, a transcription factor that we found to impose an inhibitory interneuron identity. Our work thus defines Olig3 as a key factor in cerebellar development.
Keywords:Olig3, Cerebellar Development, Cerebellar Neuron Specification, Cerebellar Hypoplasia, Fate Mapping, Neuronal Fate Change, Animals, Mice
Publisher:Cold Spring Harbor Laboratory Press
Article Number:2020.10.10.334615
Date:10 October 2020
Official Publication:https://doi.org/10.1101/2020.10.10.334615

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