RNA-binding protein Hermes/RBPMS inversely affects synapse density and axon arbor formation in retinal ganglion cells in vivo

Item Type:	Article
Title:	RNA-binding protein Hermes/RBPMS inversely affects synapse density and axon arbor formation in retinal ganglion cells in vivo
Creators Name:	Hörnberg, H. and Wollerton-van Horck, F. and Maurus, D. and Zwart, M. and Svoboda, H. and Harris, W.A. and Holt, C.E.
Abstract:	The RNA-binding protein Hermes [RNA-binding protein with multiple splicing (RBPMS)] is expressed exclusively in retinal ganglion cells (RGCs) in the CNS, but its function in these cells is not known. Here we show that Hermes protein translocates in granules from RGC bodies down the growing axons. Hermes loss of function in both Xenopus laevis and zebrafish embryos leads to a significant reduction in retinal axon arbor complexity in the optic tectum, and expression of a dominant acting mutant Hermes protein, defective in RNA-granule localization, causes similar defects in arborization. Time-lapse analysis of branch dynamics reveals that the decrease in arbor complexity is caused by a reduction in new branches rather than a decrease in branch stability. Surprisingly, Hermes depletion also leads to enhanced early visual behavior and an increase in the density of presynaptic puncta, suggesting that reduced arborization is accompanied by increased synaptogenesis to maintain synapse number.
Keywords:	Animal Behavior, Axons, Cell Differentiation, Confocal Microscopy, Cultured Cells, DNA, Electroporation, Homeostasis, Immunohistochemistry, In Situ Hybridization, Messenger RNA, Nonmammalian Embryo, Ocular Vision, Plasmids, Retina, Retinal Ganglion Cells, RNA-Binding Proteins, Synapses, Western Blotting, Xenopus Proteins, Animals, Xenopus, Zebrafish
Source:	Journal of Neuroscience
ISSN:	0270-6474
Publisher:	Society for Neuroscience
Volume:	33
Number:	25
Page Range:	10384-10395
Date:	19 June 2013
Official Publication:	https://doi.org/10.1523/JNEUROSCI.5858-12.2013
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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