Generation of three age and gender matched pairs of human induced pluripotent stem cells derived from myoblasts (MDCi011-A, MDCi012-A, MDCi013-A) and from peripheral blood mononuclear cells (MDCi011-B, MDCi012-B, MDCi013-B) from the same donor

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Item Type:	Article
Title:	Generation of three age and gender matched pairs of human induced pluripotent stem cells derived from myoblasts (MDCi011-A, MDCi012-A, MDCi013-A) and from peripheral blood mononuclear cells (MDCi011-B, MDCi012-B, MDCi013-B) from the same donor
Creators Name:	Metzler, E. and Telugu, N. and Diecke, S. and Spuler, S. and Escobar, H.
Abstract:	We describe the generation and characterization of three pairs of human induced pluripotent stem cell (hiPSC) lines reprogrammed from myoblasts and from peripheral blood mononuclear cells (PBMCs) of the same donor. All donors were free of neuromuscular disorders, female and between 47 and 50 years of age. For reprogramming we used Sendai-virus delivery of the four Yamanaka factors. The pluripotent identity of the hiPSC lines was confirmed by the expression of pluripotency markers and their capacity to differentiate into all three germ layers. These hiPSCs constitute a tool to study tissue of origin specific differences in the identity of hiPSCs.
Keywords:	Cell Differentiation, Cellular Reprogramming, Induced Pluripotent Stem Cells, Mononuclear Leukocytes, Myoblasts
Source:	Stem Cell Research
ISSN:	1873-5061
Publisher:	Elsevier
Volume:	48
Page Range:	101987
Date:	October 2020
Official Publication:	https://doi.org/10.1016/j.scr.2020.101987
PubMed:	View item in PubMed

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