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| Item Type: | Article |
|---|---|
| Title: | Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS |
| Creators Name: | Bäcker-Koduah, P. and Infante-Duarte, C. and Ivaldi, F. and Uccelli, A. and Bellmann-Strobl, J. and Wernecke, K.D. and Sy, M. and Demetriou, M. and Dörr, J. and Paul, F. and Brandt, A.U. |
| Abstract: | OBJECTIVE: To investigate the effect of cholecalciferol (vitamin D3) supplementation on peripheral immune cell frequency and N-glycan branching in patients with relapsing-remitting multiple sclerosis (RRMS). METHODS: Exploratory analysis of high-dose (20 400 IU) and low-dose (400 IU) vitamin D3 supplementation taken every other day of an 18-month randomized controlled clinical trial including 38 RRMS patients on stable immunomodulatory therapy (NCT01440062). We investigated cholecalciferol treatment effects on N-glycan branching using L-PHA stain (phaseolus vulgaris leukoagglutinin) at 6 months and frequencies of T-, B-, and NK-cell subpopulations at 12 months with flow cytometry. RESULTS: High-dose supplementation did not change CD3+ T cell subsets, CD19+ B cells subsets, and NK cells frequencies, except for CD8+ T regulatory cells, which were reduced in the low-dose arm compared to the high-dose arm at 12 months. High-dose supplementation decreased N-glycan branching on T and NK cells, measured as L-PHA mean fluorescence intensity (MFI). A reduction of N-glycan branching in B cells was not significant. In contrast, low-dose supplementation did not affect N-glycan branching. Changes in N-glycan branching did not correlate with cell frequencies. INTERPRETATION: Immunomodulatory effect of vitamin D may involve regulation of N-glycan branching in vivo. Vitamin D3 supplementation did at large not affect the frequencies of peripheral immune cells. |
| Keywords: | B-Lymphocyte Subsets, Cholecalciferol, Dietary Supplements, Immunologic Factors, Natural Killer Cells, Polysaccharides, Relapsing-Remitting Multiple Sclerosis, T-Lymphocyte Subsets, Treatment Outcome |
| Source: | Annals of Clinical and Translational Neurology |
| ISSN: | 2328-9503 |
| Publisher: | Wiley |
| Volume: | 7 |
| Number: | 9 |
| Page Range: | 1628-1641 |
| Date: | September 2020 |
| Official Publication: | https://doi.org/10.1002/acn3.51148 |
| PubMed: | View item in PubMed |
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