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Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

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Item Type:Article
Title:Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
Creators Name:Prahst, C. and Ashrafzadeh, P. and Mead, T. and Figueiredo, A. and Chang, K. and Richardson, D. and Venkaraman, L. and Richards, M. and Russo, A.M. and Harrington, K. and Ouarné, M. and Pena, A. and Chen, J.X. and Claesson-Welsh, L. and Cho, K.S. and Franco, C.A. and Bentley, K.
Abstract:As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM); 2) flat-mounting retinas for confocal microscopy significantly distorts tissue morphology, confirmed by quantitative correlative LSFM-Confocal imaging of vessels; 3) LSFM readily reveals new features of even well-studied eye disease mouse models, such as the oxygen-induced retinopathy (OIR) model, including a previously unappreciated 'knotted' morphology to pathological vascular tufts, abnormal cell motility and altered filopodia dynamics when live-imaged. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of the eye, in particular pathological, neurovascular, degenerative processes.
Keywords:Confocal Microscopy, Eye Diseases, Fluorescence Microscopy, Retina, Retinal Vessels, Three-Dimensional Imaging, Animals, Mice
Publisher:eLife Sciences Publications
Page Range:e49779
Date:19 February 2020
Official Publication:https://doi.org/10.7554/eLife.49779
PubMed:View item in PubMed

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