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Quantitative imaging of gut microbiota spatial organization

Item Type:Article
Title:Quantitative imaging of gut microbiota spatial organization
Creators Name:Earle, K.A. and Billings, G. and Sigal, M. and Lichtman, J.S. and Hansson, G.C. and Elias, J.E. and Amieva, M.R. and Huang, K.C. and Sonnenburg, J.L.
Abstract:Genomic technologies have significantly advanced our understanding of the composition and diversity of host-associated microbial populations. However, their spatial organization and functional interactions relative to the host have been more challenging to study. Here we present a pipeline for the assessment of intestinal microbiota localization within immunofluorescence images of fixed gut cross-sections that includes a flexible software package, BacSpace, for high-throughput quantification of microbial organization. Applying this pipeline to gnotobiotic and human microbiota-colonized mice, we demonstrate that elimination of microbiota-accessible carbohydrates (MACs) from the diet results in thinner mucus in the distal colon, increased proximity of microbes to the epithelium, and heightened expression of the inflammatory marker REG3β. Measurements of microbe-microbe proximity reveal that a MAC-deficient diet alters monophyletic spatial clustering. Furthermore, we quantify the invasion of Helicobacter pylori into the glands of the mouse stomach relative to host mitotic progenitor cells, illustrating the generalizability of this approach.
Keywords:Bacteriological Techniques, Carbohydrate Metabolism, Diet, Fluorescence Microscopy, Gastrointestinal Microbiome, Gastrointestinal Tract, Helicobacter pylori, Host-Pathogen Interactions, Optical Imaging, Animals, Mice
Source:Cell Host & Microbe
Publisher:Elsevier / Cell Press
Page Range:478-488
Date:14 October 2015
Official Publication:https://doi.org/10.1016/j.chom.2015.09.002
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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