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Endogenous retrovirus-derived lncRNA BANCR promotes cardiomyocyte migration in humans and non-human primates

Item Type:Article
Title:Endogenous retrovirus-derived lncRNA BANCR promotes cardiomyocyte migration in humans and non-human primates
Creators Name:Wilson, K.D. and Ameen, M. and Guo, H. and Abilez, O.J. and Tian, L. and Mumbach, M.R. and Diecke, S. and Qin, X. and Liu, Y. and Yang, H. and Ma, N. and Gaddam, S. and Cunningham, N.J. and Gu, M. and Neofytou, E. and Prado, M. and Hildebrandt, T.B. and Karakikes, I. and Chang, H.Y. and Wu, J.C.
Abstract:Transposable elements (TEs) comprise nearly half of the human genome and are often transcribed or exhibit cis-regulatory properties with unknown function in specific processes such as heart development. In the case of endogenous retroviruses (ERVs), a TE subclass, experimental interrogation is constrained as many are primate-specific or human-specific. Here, we use primate pluripotent stem-cell-derived cardiomyocytes that mimic fetal cardiomyocytes in vitro to discover hundreds of ERV transcripts from the primate-specific MER41 family, some of which are regulated by the cardiogenic transcription factor TBX5. The most significant of these are located within BANCR, a long non-coding RNA (lncRNA) exclusively expressed in primate fetal cardiomyocytes. Functional studies reveal that BANCR promotes cardiomyocyte migration in vitro and ventricular enlargement in vivo. We conclude that recently evolved TE loci such as BANCR may represent potent de novo developmental regulatory elements that can be interrogated with species-matching pluripotent stem cell models.
Keywords:Induced Pluripotent Stem Cells, Transposable Elements, Endogenous Retroviruses, lncRNAs, Migration, Cardiomyocyte, Heart Development, Primate, Evolution, Animals, Primates
Source:Developmental Cell
Publisher:Cell Press
Page Range:694-709
Date:28 September 2020
Additional Information:Copyright © 2020 Elsevier Inc.
Official Publication:https://doi.org/10.1016/j.devcel.2020.07.006
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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