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Functional annotation of human long noncoding RNAs via molecular phenotyping

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Item Type:Article
Title:Functional annotation of human long noncoding RNAs via molecular phenotyping
Creators Name:Ramilowski, J.A. and Yip, C.W. and Agrawal, S. and Chang, J.C. and Ciani, Y. and Kulakovskiy, I.V. and Mendez, M. and Ooi, J.L.C. and Ouyang, J.F. and Parkinson, N. and Petri, A. and Roos, L. and Severin, J. and Yasuzawa, K. and Abugessaisa, I. and Akalin, A. and Antonov, I.V. and Arner, E. and Bonetti, A. and Bono, H. and Borsari, B. and Brombacher, F. and Cameron, C.J.F. and Cannistraci, C.V. and Cardenas, R. and Cardon, M. and Chang, H. and Dostie, J. and Ducoli, L. and Favorov, A. and Fort, A. and Garrido, D. and Gil, N. and Gimenez, J. and Guler, R. and Handoko, L. and Harshbarger, J. and Hasegawa, A. and Hasegawa, Y. and Hashimoto, K. and Hayatsu, N. and Heutink, P. and Hirose, T. and Imada, E.L. and Itoh, M. and Kaczkowski, B. and Kanhere, A. and Kawabata, E. and Kawaji, H. and Kawashima, T. and Kelly, S.T. and Kojima, M. and Kondo, N. and Koseki, H. and Kouno, T. and Kratz, A. and Kurowska-Stolarska, M. and Kwon, A.T.J. and Leek, J. and Lennartsson, A. and Lizio, M. and López-Redondo, F. and Luginbühl, J. and Maeda, S. and Makeev, V.J. and Marchionni, L. and Medvedeva, Y.A. and Minoda, A. and Müller, F. and Muñoz-Aguirre, M. and Murata, M. and Nishiyori, H. and Nitta, K.R. and Noguchi, S. and Noro, Y. and Nurtdinov, R. and Okazaki, Y. and Orlando, V. and Paquette, D. and Parr, C.J.C. and Rackham, O.J.L. and Rizzu, P. and Sánchez Martinez, D.F. and Sandelin, A. and Sanjana, P. and Semple, C.A.M. and Shibayama, Y. and Sivaraman, D.M. and Suzuki, T. and Szumowski, S.C. and Tagami, M. and Taylor, M.S. and Terao, C. and Thodberg, M. and Thongjuea, S. and Tripathi, V. and Ulitsky, I. and Verardo, R. and Vorontsov, I.E. and Yamamoto, C. and Young, R.S. and Baillie, J.K. and Forrest, A.R.R. and Guigó, R. and Hoffman, M.M. and Hon, C.C. and Kasukawa, T. and Kauppinen, S. and Kere, J. and Lenhard, B. and Schneider, C. and Suzuki, H. and Yagi, K. and de Hoon, M.J.L. and Shin, J.W. and Carninci, P.
Abstract:Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
Keywords:Antisense Oligonucleotides, Cell Growth Processes, Cell Movement, Fibroblasts, KCNQ Potassium Channels, Long Noncoding RNA, Molecular Sequence Annotation, Small Interfering RNA
Source:Genome Research
Publisher:Cold Spring Harbor Laboratory Press
Page Range:1060-1072
Date:July 2020
Additional Information:Erratum in: Genome Res 30(9):1377-1.
Official Publication:https://doi.org/10.1101/gr.254219.119
PubMed:View item in PubMed
Related to:
https://edoc.mdc-berlin.de/18641/Preprint version

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