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Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

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Item Type:Article
Title:Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke
Creators Name:Ihantola, T. and Di Bucchianico, S. and Happo, M. and Ihalainen, M. and Uski, O. and Bauer, S. and Kuuspalo, K. and Sippula, O. and Tissari, J. and Oeder, S. and Hartikainen, A. and Rönkkö, T.J. and Martikainen, M.V. and Huttunen, K. and Vartiainen, P. and Suhonen, H. and Kortelainen, M. and Lamberg, H. and Leskinen, A. and Sklorz, M. and Michalke, B. and Dilger, M. and Weiss, C. and Dittmar, G. and Beckers, J. and Irmler, M. and Buters, J. and Candeias, J. and Czech, H. and Yli-Pirilä, P. and Abbaszade, G. and Jakobi, G. and Orasche, J. and Schnelle-Kreis, J. and Kanashova, T. and Karg, E. and Streibel, T. and Passig, J. and Hakkarainen, H. and Jokiniemi, J. and Zimmermann, R. and Hirvonen, M.R. and Jalava, P.I.
Abstract:BACKGROUND: Wood combustion emissions have been studied previously either by in vitro or in vivo models using collected particles, yet most studies have neglected gaseous compounds. Furthermore, a more accurate and holistic view of the toxicity of aerosols can be gained with parallel in vitro and in vivo studies using direct exposure methods. Moreover, modern exposure techniques such as air-liquid interface (ALI) exposures enable better assessment of the toxicity of the applied aerosols than, for example, the previous state-of-the-art submerged cell exposure techniques. METHODS: We used three different ALI exposure systems in parallel to study the toxicological effects of spruce and pine combustion emissions in human alveolar epithelial (A549) and murine macrophage (RAW264.7) cell lines. A whole-body mouse inhalation system was also used to expose C57BL/6 J mice to aerosol emissions. Moreover, gaseous and particulate fractions were studied separately in one of the cell exposure systems. After exposure, the cells and animals were measured for various parameters of cytotoxicity, inflammation, genotoxicity, transcriptome and proteome. RESULTS: We found that diluted (1:15) exposure pine combustion emissions (PM(1) mass 7.7 ± 6.5 mg m(-3), 41 mg MJ(-1)) contained, on average, more PM and polycyclic aromatic hydrocarbons (PAHs) than spruce (PM(1) mass 4.3 ± 5.1 mg m(-3), 26 mg MJ(-1)) emissions, which instead showed a higher concentration of inorganic metals in the emission aerosol. Both A549 cells and mice exposed to these emissions showed low levels of inflammation but significantly increased genotoxicity. Gaseous emission compounds produced similar genotoxicity and a higher inflammatory response than the corresponding complete combustion emission in A549 cells. Systems biology approaches supported the findings, but we detected differing responses between in vivo and in vitro experiments. CONCLUSIONS: Comprehensive in vitro and in vivo exposure studies with emission characterization and systems biology approaches revealed further information on the effects of combustion aerosol toxicity than could be achieved with either method alone. Interestingly, in vitro and in vivo exposures showed the opposite order of the highest DNA damage. In vitro measurements also indicated that the gaseous fraction of emission aerosols may be more important in causing adverse toxicological effects. Combustion aerosols of different wood species result in mild but aerosol specific in vitro and in vivo effects.
Keywords:Particulate Matter (PM), Air Liquid-Interface (ALI), Inhalation Toxicology, Wood Combustion, Transcriptome, Proteome, Genotoxicity, Animals, Mice
Source:Particle and Fibre Toxicology
Publisher:BioMed Central
Page Range:27
Date:15 June 2020
Official Publication:https://doi.org/10.1186/s12989-020-00355-1
PubMed:View item in PubMed

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