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Phosphodiesterase 3A and arterial hypertension

Item Type:Article
Title:Phosphodiesterase 3A and arterial hypertension
Creators Name:Ercu, M. and Markó, L. and Schächterle, C. and Tsvetkov, D. and Cui, Y. and Maghsodi, S. and Bartolomaeus, T.U.P. and Maass, P.G. and Zühlke, K. and Gregersen, N. and Hübner, N. and Hodge, R. and Mühl, A. and Pohl, B. and Molé-Illas, R. and Geelhaar, A. and Walter, S. and Napieczynska, H. and Schelenz, S. and Taube, M. and Heuser, A. and Anistan, Y.M. and Qadri, F. and Todiras, M. and Plehm, R. and Popova, E. and Langanki, R. and Eichhorst, J. and Lehmann, M. and Wiesner, B. and Russwurm, M. and Forslund, S.K. and Kamer, I. and Müller, D.N. and Gollasch, M. and Aydin, A. and Bähring, S. and Bader, M. and Luft, F.C. and Klussmann, E.
Abstract:BACKGROUND: High blood pressure is the primary risk factor for cardiovascular death worldwide. Autosomal-dominant hypertension with brachydactyly (HTNB) clinically resembles salt-resistant essential hypertension and causes death by stroke before age 50 years. Recently, we implicated the gene encoding phosphodiesterase 3A (PDE3A); however, in vivo modeling of the genetic defect and thus showing an involvement of mutant PDE3A is lacking. METHODS: We used genetic mapping, sequencing, transgenic technology, CRISPR-Cas9 gene editing, immunoblotting, and fluorescence resonance energy transfer (FRET). We identified new patients, performed extensive animal phenotyping, and explored new signaling pathways. RESULTS: We describe a novel mutation within a 15 bp region of the PDE3A gene and define this segment as mutational hotspot in HTNB. The mutations cause an increase in enzyme activity. A CRISPR/Cas9-generated rat model, with a 9 bp deletion within the hotspot analogous to a human deletion, recapitulates HTNB. In mice, mutant transgenic PDE3A overexpression in smooth muscle cells confirmed that mutant PDE3A causes hypertension. The mutant PDE3A enzymes display consistent changes in their phosphorylation and an increased interaction with the 14-3-3θ adaptor protein. This aberrant signaling is associated with an increase in vascular smooth muscle cell proliferation and changes in vessel morphology and function. CONCLUSIONS: The mutated PDE3A gene drives mechanisms that increase peripheral vascular resistance causing hypertension. We present two new animal models that will serve to elucidate the underlying mechanisms further. Our findings could facilitate the search for new antihypertensive treatments.
Keywords:Hypertension, Genetics, Blood Pressure Regulation, Phosphodiesterase, Animals, Rats
Source:Circulation
ISSN:0009-7322
Publisher:American Heart Association
Volume:142
Number:2
Page Range:133-149
Date:14 July 2020
Official Publication:https://doi.org/10.1161/CIRCULATIONAHA.119.043061
PubMed:View item in PubMed

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