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Afadin signaling at the spinal neuroepithelium regulates central canal formation and gait selection

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Item Type:Article
Title:Afadin signaling at the spinal neuroepithelium regulates central canal formation and gait selection
Creators Name:Skarlatou, S. and Hérent, C. and Toscano, E. and Mendes, C.S. and Bouvier, J. and Zampieri, N.
Abstract:Afadin, a scaffold protein controlling the activity of the nectin family of cell adhesion molecules, regulates important morphogenetic processes during development. In the central nervous system, afadin has critical roles in neuronal migration, axonal elongation, and synapse formation. Here we examine the role of afadin in development of spinal motor circuits. Afadin elimination in motor neuron progenitors results in striking locomotor behavior: left-right limb alternation is substituted by synchronous activation, characteristic of bound gait. We find that afadin function at the neuroepithelium is required for structural organization of the spinal midline and central canal morphogenesis. Perturbation of afadin results in formation of two central canals, aberrant contralateral wiring of different classes of spinal premotor interneurons, and loss of left-right limb alternation, highlighting important developmental principles controlling the assembly of spinal motor circuits.
Keywords:Afadin, Spinal Cord, Locomotor Circuits, Premotor Connectivity, Gait, Spinal Interneurons, Central Canal Development, Animals, Mice
Source:Cell Reports
Publisher:Cell Press / Elsevier
Page Range:107741
Date:9 June 2020
Additional Information:Erratum in: Cell Rep 34(4):108671.
Official Publication:https://doi.org/10.1016/j.celrep.2020.107741
PubMed:View item in PubMed

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