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Peptide-based interaction proteomics

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Item Type:Review
Title:Peptide-based interaction proteomics
Creators Name:Meyer, K. and Selbach, M.
Abstract:Protein-protein interactions are often mediated by short linear motifs (SLiMs) that are located in intrinsically disordered regions (IDRs) of proteins. Interactions mediated by SLiMs are notoriously difficult to study, and many functionally relevant interactions likely remain to be uncovered. Recently, pull-downs with synthetic peptides in combination with quantitative mass spectrometry emerged as a powerful screening approach to study protein-protein interactions mediated by SLiMs. Specifically, arrays of synthetic peptides immobilized on cellulose membranes provide a scalable means to identify the interaction partners of many peptides in parallel. In this minireview we briefly highlight the relevance of SLiMs for protein-protein interactions, outline existing screening technologies, discuss unique advantages of peptide-based interaction screens and provide practical suggestions for setting up such peptide-based screens.
Keywords:Intrinsically Disordered Regions, Short Linear Motifs, Affinity Proteomics, High Throughput Screening, Mass Spectrometry, Peptide Array, Peptide Interactions, Peptides, Post-Translational Modifications, Protein-Protein Interactions
Source:Molecular & Cellular Proteomics
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:1070-1075
Date:July 2020
Official Publication:https://doi.org/10.1074/mcp.R120.002034
PubMed:View item in PubMed

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