Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Ribosome profiling at isoform level reveals evolutionary conserved impacts of differential splicing on the proteome

[img]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1MB
[img] Other (Supplementary Information)
50MB

Item Type:Article
Title:Ribosome profiling at isoform level reveals evolutionary conserved impacts of differential splicing on the proteome
Creators Name:Reixachs-Solé, M. and Ruiz-Orera, J. and Albà, M.M. and Eyras, E.
Abstract:The differential production of transcript isoforms from gene loci is a key cellular mechanism. Yet, its impact in protein production remains an open question. Here, we describe ORQAS (ORF quantification pipeline for alternative splicing), a pipeline for the translation quantification of individual transcript isoforms using ribosome-protected mRNA fragments (ribosome profiling). We find evidence of translation for 40-50% of the expressed isoforms in human and mouse, with 53% of the expressed genes having more than one translated isoform in human, and 33% in mouse. Differential splicing analysis revealed that about 40% of the splicing changes at RNA level are concordant with changes in translation. Furthermore, orthologous cassette exons between human and mouse preserve the directionality of the change, and are enriched in microexons in a comparison between glia and glioma. ORQAS leverages ribosome profiling to uncover a widespread and evolutionarily conserved impact of differential splicing on translation, particularly of microexon-containing isoforms.
Keywords:Animals, Mice
Source:Nature Communications
ISSN:2041-1723
Publisher:Nature Publishing Group
Volume:11
Number:1
Page Range:1768
Date:14 April 2020
Official Publication:https://doi.org/10.1038/s41467-020-15634-w
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library