Pandemic 2009 H1N1 influenza A virus carrying a Q136K mutation in the neuraminidase gene is resistant to zanamivir but exhibits reduced fitness in the guinea pig transmission model

Item Type:	Article
Title:	Pandemic 2009 H1N1 influenza A virus carrying a Q136K mutation in the neuraminidase gene is resistant to zanamivir but exhibits reduced fitness in the guinea pig transmission model
Creators Name:	Kaminski, M.M. and Ohnemus, A. and Staeheli, P. and Rubbenstroth, D.
Abstract:	Resistance of influenza A viruses to neuraminidase inhibitors can arise through mutations in the neuraminidase (NA) gene. We show here that a Q136K mutation in the NA of the 2009 pandemic H1N1 virus confers a high degree of resistance to zanamivir. Resistance is accompanied by reduced numbers of NA molecules in viral particles and reduced intrinsic enzymatic activity of mutant NA. Interestingly, the Q136K mutation strongly impairs viral fitness in the guinea pig transmission model.
Keywords:	Amino Acid Substitution, Animal Disease Models, Antiviral Agents, DNA Sequence Analysis, H1N1 Subtype Influenza A Virus, Missense Mutation, Molecular Sequence Data, Mutant Proteins, Neuraminidase, Orthomyxoviridae Infections, Viral Drug Resistance, Viral Proteins, Viral RNA, Virulence, Virulence Factors, Zanamivir, Animals, Guinea Pigs
Source:	Journal of Virology
ISSN:	0022-538X
Publisher:	American Society for Microbiology
Volume:	87
Number:	3
Page Range:	1912-1915
Date:	February 2013
Official Publication:	https://doi.org/10.1128/JVI.02507-12
PubMed:	View item in PubMed

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