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A highly conserved circular RNA is required to keep neural cells in a progenitor state in the mammalian brain

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Item Type:Article
Title:A highly conserved circular RNA is required to keep neural cells in a progenitor state in the mammalian brain
Creators Name:Suenkel, C. and Cavalli, D. and Massalini, S. and Calegari, F. and Rajewsky, N.
Abstract:circSLC45A4 is the main RNA splice isoform produced from its genetic locus and one of the highest expressed circRNAs in the developing human frontal cortex. Knockdown of this highly conserved circRNA in a human neuroblastoma cell line is sufficient to induce spontaneous neuronal differentiation, measurable by increased expression of neuronal marker genes. Depletion of circSlc45a4 in the developing mouse cortex causes a significant reduction of the basal progenitor pool and increases the expression of neurogenic regulators. Furthermore, knockdown of circSlc45a4a induces a significant depletion of cells in the cortical plate. In addition, deconvolution of the bulk RNA-seq data with the help of single-cell RNA-seq data validates the depletion of basal progenitors and reveals an increase in Cajal-Retzius cells. In summary, we present a detailed study of a highly conserved circular RNA that is necessary to maintain the pool of neural progenitors in vitro and in vivo.
Keywords:Circular RNA, circRNA, Neurogenesis, Cortical Development, Neuronal Differentiation, Basal Progenitor, RNA-seq, Animals, Mice
Source:Cell Reports
ISSN:2211-1247
Publisher:Cell Press / Elsevier
Volume:30
Number:7
Page Range:2170-2179
Date:18 February 2020
Official Publication:https://doi.org/10.1016/j.celrep.2020.01.083
PubMed:View item in PubMed

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