Helmholtz Gemeinschaft


Low-density granulocytes are a novel immunopathological feature in both multiple sclerosis and neuromyelitis optica spectrum disorder

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Item Type:Article
Title:Low-density granulocytes are a novel immunopathological feature in both multiple sclerosis and neuromyelitis optica spectrum disorder
Creators Name:Ostendorf, L. and Mothes, R. and van Koppen, S. and Lindquist, R.L. and Bellmann-Strobl, J. and Asseyer, S. and Ruprecht, K. and Alexander, T. and Niesner, R.A. and Hauser, A.E. and Paul, F. and Radbruch, H.
Abstract:OBJECTIVE: To investigate whether low-density granulocytes (LDGs) are an immunophenotypic feature of patients with multiple sclerosis (MS) or neuromyelitis optica spectrum disorder (NMOSD). METHODS: Blood samples were collected from 20 patients with NMOSD and 17 patients with MS, as well as from 15 patients with Systemic Lupus Erythematosus (SLE) and 23 Healthy Donors (HD). We isolated peripheral blood mononuclear cells (PBMCs) with density gradient separation and stained the cells with antibodies against CD14, CD15, CD16, and CD45, and analyzed the cells by flow cytometry or imaging flow cytometry. We defined LDGs as CD14-CD15(high) and calculated their share in total PBMC leukocytes (CD45(+)) as well as the share of CD16(hi) LDGs. Clinical data on disease course, medication, and antibody status were obtained. RESULTS: LDGs were significantly more common in MS and NMOSD than in HDs, comparable to SLE samples (median values HD 0.2%, MS 0.9%, NMOSD 2.1%, SLE 4.3%). 0/23 of the HDs, but 17/20 NMOSD and 11/17 MS samples as well as 13/15 SLE samples had at least 0.7 % LDGs. NMOSD patients without continuous immunosuppressive treatment had significantly more LDGs compared to their treated counterparts. LDG nuclear morphology ranged from segmented to rounded, suggesting a heterogeneity within the group. CONCLUSION: LDGs are a feature of the immunophenotype in some patients with MS and NMOSD.
Keywords:Low-Density Granulocytes, Multiple Sclerosis, Neuromyelitis Optica, Neuroinflammation, Autoimmunity
Source:Frontiers in Immunology
Publisher:Frontiers Media SA
Page Range:2725
Date:29 November 2019
Official Publication:https://doi.org/10.3389/fimmu.2019.02725
PubMed:View item in PubMed
Related to:
https://edoc.mdc-berlin.de/18322/Preprint version

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library