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| Item Type: | Article |
|---|---|
| Title: | Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog |
| Creators Name: | Popowski, M. and Templeton, T.D. and Lee, B.K. and Rhee, C. and Li, H. and Miner, C. and Dekker, J.D. and Orlanski, S. and Bergman, Y. and Iyer, V.R. and Webb, C.F. and Tucker, H. |
| Abstract: | We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming of mouse embryonic fibroblasts (MEFs) and enhancement of standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under standard embryonic stem cell (ESC) culture conditions, spontaneously form clones that in vitro express pluripotency markers, differentiate to all germ lineages, and in vivo form teratomas and chimeric mice. We demonstrate that BRIGHT binds directly to the promoter/enhancer regions of Oct4, Sox2, and Nanog to contribute to their repression in both MEFs and ESCs. Thus, elimination of the BRIGHT barrier may provide an approach for somatic cell reprogramming. |
| Keywords: | Cell Line, Cellular Reprogramming, Cellular Senescence, DNA-Binding Proteins, Genetic Promoter Regions, Homeodomain Proteins, Lewis X Antigen, Nanog Homeobox Protein, Octamer Transcription Factor-3, Protein Binding, RNA Interference, Small Interfering RNA, SOXB1 Transcription Factors, Transcription Factors, Transcriptome, Animals, Mice |
| Source: | Stem Cell Reports |
| ISSN: | 2213-6711 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 2 |
| Number: | 1 |
| Page Range: | 26-35 |
| Date: | 14 January 2014 |
| Official Publication: | https://doi.org/10.1016/j.stemcr.2013.12.002 |
| PubMed: | View item in PubMed |
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