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Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons

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Item Type:Article
Title:Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons
Creators Name:Sampathkumar, C. and Wu, Y.J. and Vadhvani, M. and Trimbuch, T. and Eickholt, B. and Rosenmund, C.
Abstract:Mutations in the MECP2 gene cause the neurodevelopmental disorder Rett syndrome (RTT). Previous studies have shown that altered MeCP2 levels result in aberrant neurite outgrowth and glutamatergic synapse formation. However, causal molecular mechanisms are not well understood since MeCP2 is known to regulate transcription of a wide range of target genes. Here, we describe a key role for a constitutive BDNF feed forward signaling pathway in regulating synaptic response, general growth and differentiation of glutamatergic neurons. Chronic block of TrkB receptors mimics the MeCP2 deficiency in wildtype glutamatergic neurons, while re-expression of BDNF quantitatively rescues MeCP2 deficiency. We show that BDNF acts cell autonomous and autocrine, as wildtype neurons are not capable of rescuing growth deficits in neighboring MeCP2 deficient neurons in vitro and in vivo. These findings are relevant for understanding RTT pathophysiology, wherein wildtype and mutant neurons are intermixed throughout the nervous system.
Keywords:BDNF, Rett Syndrome, Cell Autonomous, Glutamatergic, Neuroscience, Synaptic Transmission, Animals, Mice
Publisher:eLife Sciences Publications
Page Range:e19374
Date:26 October 2016
Official Publication:https://doi.org/10.7554/eLife.19374
PubMed:View item in PubMed

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