Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

High-resolution chromatin dynamics during a yeast stress response

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
7MB
[thumbnail of Supplemental Information] Other (Supplemental Information)
69MB

Item Type:Article
Title:High-resolution chromatin dynamics during a yeast stress response
Creators Name:Weiner, A., Hsieh, T.H.S., Appleboim, A., Chen, H.V., Rahat, A., Amit, I., Rando, O.J. and Friedman, N.
Abstract:Covalent histone modifications are highly conserved and play multiple roles in eukaryotic transcription regulation. Here, we mapped 26 histone modifications genome-wide in exponentially growing yeast and during a dramatic transcriptional reprogramming-the response to diamide stress. We extend prior studies showing that steady-state histone modification patterns reflect genomic processes, especially transcription, and display limited combinatorial complexity. Interestingly, during the stress response we document a modest increase in the combinatorial complexity of histone modification space, resulting from roughly 3% of all nucleosomes transiently populating rare histone modification states. Most of these rare histone states result from differences in the kinetics of histone modification that transiently uncouple highly correlated marks, with slow histone methylation changes often lagging behind the more rapid acetylation changes. Explicit analysis of modification dynamics uncovers ordered sequences of events in gene activation and repression. Together, our results provide a comprehensive view of chromatin dynamics during a massive transcriptional upheaval.
Keywords:Chromatin, Chromatin Assembly and Disassembly, Diamide, Fungal Gene Expression Regulation, Fungal Genome, Genetic Transcription, Histones, Molecular Sequence Data, Saccharomyces Cerevisiae
Source:Molecular Cell
ISSN:1097-2765
Publisher:Cell Press
Volume:58
Number:2
Page Range:371-386
Date:16 April 2015
Official Publication:https://doi.org/10.1016/j.molcel.2015.02.002
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library