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A synthetic biology approach to probing nucleosome symmetry

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Item Type:Article
Title:A synthetic biology approach to probing nucleosome symmetry
Creators Name:Ichikawa, Y. and Connelly, C.F. and Appleboim, A. and Miller, T.C. and Jacobi, H. and Abshiru, N.A. and Chou, H.J. and Chen, Y. and Sharma, U. and Zheng, Y. and Thomas, P.M. and Chen, H.V. and Bajaj, V. and Müller, C.W. and Kelleher, N.L. and Friedman, N. and Bolon, D.N.A. and Rando, O.J. and Kaufman, P.D.
Abstract:The repeating subunit of chromatin, the nucleosome, includes two copies of each of the four core histones, and several recent studies have reported that asymmetrically-modified nucleosomes occur at regulatory elements in vivo. To probe the mechanisms by which histone modifications are read out, we designed an obligate pair of H3 heterodimers, termed H3X and H3Y, which we extensively validated genetically and biochemically. Comparing the effects of asymmetric histone tail point mutants with those of symmetric double mutants revealed that a single methylated H3K36 per nucleosome was sufficient to silence cryptic transcription in vivo. We also demonstrate the utility of this system for analysis of histone modification crosstalk, using mass spectrometry to separately identify modifications on each H3 molecule within asymmetric nucleosomes. The ability to generate asymmetric nucleosomes in vivo and in vitro provides a powerful and generalizable tool to probe the mechanisms by which H3 tails are read out by effector proteins in the cell.
Keywords:Histones, Mass Spectrometry, Nucleosomes, Post-Translational Protein Processing, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Synthetic Biology
Publisher:eLife Sciences Publications
Page Range:e28836
Date:12 September 2017
Official Publication:https://doi.org/10.7554/eLife.28836.001
PubMed:View item in PubMed

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