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The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant

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Item Type:Article
Title:The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant
Creators Name:Bogdanow, B. and Wang, X. and Eichelbaum, K. and Sadewasser, A. and Husic, I. and Paki, K. and Budt, M. and Hergeselle, M. and Vetter, B. and Hou, J. and Chen, W. and Wiebusch, L. and Meyer, I.M. and Wolff, T. and Selbach, M.
Abstract:Pandemic influenza A virus (IAV) outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular basis of this species barrier is incompletely understood. Here we combine metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon infection of human cells with a human- and a bird-adapted IAV strain and observe striking differences in viral protein synthesis. Most importantly, the matrix protein M1 is inefficiently produced by the bird-adapted strain. We show that impaired production of M1 from bird-adapted strains is caused by increased splicing of the M segment RNA to alternative isoforms. Strain-specific M segment splicing is controlled by the 3' splice site and functionally important for permissive infection. In silico and biochemical evidence shows that avian-adapted M segments have evolved different conserved RNA structure features than human-adapted sequences. Thus, we identify M segment RNA splicing as a viral host range determinant.
Keywords:HEK293 Cells, Host Specificity, Human Influenza, Influenza A Virus, Madin Darby Canine Kidney Cells, Orthomyxoviridae Infections, Proteome, Proteomics, RNA Splicing, Viral Matrix Proteins, Animals, Birds, Dogs
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:5518
Date:4 December 2019
Official Publication:https://doi.org/10.1038/s41467-019-13520-8
PubMed:View item in PubMed
Related to:
https://edoc.mdc-berlin.de/17802/Preprint version

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