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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Item Type:Article
Title:Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Creators Name:Cossarizza, A. and Chang, H.D. and Radbruch, A. and Acs, A. and Adam, D. and Adam-Klages, S. and Agace, W.W. and Aghaeepour, N. and Akdis, M. and Allez, M. and Almeida, L.N. and Alvisi, G. and Anderson, G. and Andrä, I. and Annunziato, F. and Anselmo, A. and Bacher, P. and Baldari, C.T. and Bari, S. and Barnaba, V. and Barros-Martins, J. and Battistini, L. and Bauer, W. and Baumgart, S. and Baumgarth, N. and Baumjohann, D. and Baying, B. and Bebawy, M. and Becher, B. and Beisker, W. and Benes, V. and Beyaert, R. and Blanco, A. and Boardman, D.A. and Bogdan, C. and Borger, J.G. and Borsellino, G. and Boulais, P.E. and Bradford, J.A. and Brenner, D. and Brinkman, R.R. and Brooks, A.E.S. and Busch, D.H. and Büscher, M. and Bushnell, T.P. and Calzetti, F. and Cameron, G. and Cammarata, I. and Cao, X. and Cardell, S.L. and Casola, S. and Cassatella, M.A. and Cavani, A. and Celada, A. and Chatenoud, L. and Chattopadhyay, P.K. and Chow, S. and Christakou, E. and Čičin-Šain, L. and Clerici, M. and Colombo, F.S. and Cook, L. and Cooke, A. and Cooper, A.M. and Corbett, A.J. and Cosma, A. and Cosmi, L. and Coulie, P.G. and Cumano, A. and Cvetkovic, L. and Dang, V.D. and Dang-Heine, C. and Davey, M.S. and Davies, D. and De Biasi, S. and Del Zotto, G. and Dela Cruz, G.V. and Delacher, M. and Della Bella, S. and Dellabona, P. and Deniz, G. and Dessing, M. and Di Santo, J.P. and Diefenbach, A. and Dieli, F. and Dolf, A. and Dörner, T. and Dress, R.J. and Dudziak, D. and Dustin, M. and Dutertre, C.A. and Ebner, F. and Eckle, S.B.G. and Edinger, M. and Eede, P. and Ehrhardt, G.R.A. and Eich, M. and Engel, P. and Engelhardt, B. and Erdei, A. and Esser, C. and Everts, B. and Evrard, M. and Falk, C.S. and Fehniger, T.A. and Felipo-Benavent, M. and Ferry, H. and Feuerer, M. and Filby, A. and Filkor, K. and Fillatreau, S. and Follo, M. and Förster, I. and Foster, J. and Foulds, G.A. and Frehse, B. and Frenette, P.S. and Frischbutter, S. and Fritzsche, W. and Galbraith, D.W. and Gangaev, A. and Garbi, N. and Gaudilliere, B. and Gazzinelli, R.T. and Geginat, J. and Gerner, W. and Gherardin, N.A. and Ghoreschi, K. and Gibellini, L. and Ginhoux, F. and Goda, K. and Godfrey, D.I. and Goettlinger, C. and González-Navajas, J.M. and Goodyear, C.S. and Gori, A. and Grogan, J.L. and Grummitt, D. and Grützkau, A. and Haftmann, C. and Hahn, J. and Hammad, H. and Hämmerling, G. and Hansmann, L. and Hansson, G. and Harpur, C.M. and Hartmann, S. and Hauser, A. and Hauser, A.E. and Haviland, D.L. and Hedley, D. and Hernández, D.C. and Herrera, G. and Herrmann, M. and Hess, C. and Höfer, T. and Hoffmann, P. and Hogquist, K. and Holland, T. and Höllt, T. and Holmdahl, R. and Hombrink, P. and Houston, J.P. and Hoyer, B.F. and Huang, B. and Huang, F.P. and Huber, J.E. and Huehn, J. and Hundemer, M. and Hunter, C.A. and Hwang, W.Y.K. and Iannone, A. and Ingelfinger, F. and Ivison, S.M. and Jäck, H.M. and Jani, P.K. and Jávega, B. and Jonjic, S. and Kaiser, T. and Kalina, T. and Kamradt, T. and Kaufmann, S.H.E. and Keller, B. and Ketelaars, S.L.C. and Khalilnezhad, A. and Khan, S. and Kisielow, J. and Klenerman, P. and Knopf, J. and Koay, H.F. and Kobow, K. and Kolls, J.K. and Kong, W.T. and Kopf, M. and Korn, T. and Kriegsmann, K. and Kristyanto, H. and Kroneis, T. and Krueger, A. and Kühne, J. and Kukat, C. and Kunkel, D. and Kunze-Schumacher, H. and Kurosaki, T. and Kurts, C. and Kvistborg, P. and Kwok, I. and Landry, J. and Lantz, O. and Lanuti, P. and LaRosa, F. and Lehuen, A. and LeibundGut-Landmann, S. and Leipold, M.D. and Leung, L.Y.T. and Levings, M.K. and Lino, A.C. and Liotta, F. and Litwin, V. and Liu, Y. and Ljunggren, H.G. and Lohoff, M. and Lombardi, G. and Lopez, L. and López-Botet, M. and Lovett-Racke, A.E. and Lubberts, E. and Luche, H. and Ludewig, B. and Lugli, E. and Lunemann, S. and Maecker, H.T. and Maggi, L. and Maguire, O. and Mair, Fl. and Mair, K.H. and Mantovani, A. and Manz, R.A. and Marshall, A.J. and Martínez-Romero, A. and Martrus, G. and Marventano, I. and Maslinski, W. and Matarese, G. and Mattioli, A.V. and Maueröder, C. and Mazzoni, A. and McCluskey, J. and McGrath, M. and McGuire, H.M. and McInnes, I.B. and Mei, H.E. and Melchers, F. and Melzer, S. and Mielenz, D. and Miller, S.D. and Mills, K.H.G. and Minderman, H. and Mjösberg, J. and Moore, J. and Moran, B. and Moretta, L. and Mosmann, T.R. and Müller, S. and Multhoff, G. and Muñoz, L.E. and Münz, C. and Nakayama, T. and Nasi, M. and Neumann, K. and Ng, L.G. and Niedobitek, A. and Nourshargh, S. and Núñez, G. and O'Connor, J.E. and Ochel, A. and Oja, A. and Ordonez, D. and Orfao, A. and Orlowski-Oliver, E. and Ouyang, W. and Oxenius, A. and Palankar, R. and Panse, I. and Pattanapanyasat, K. and Paulsen, M. and Pavlinic, D. and Penter, L. and Peterson, P. and Peth, C. and Petriz, J. and Piancone, F. and Pickl, W.F. and Piconese, S. and Pinti, M. and Pockley, A.G. and Podolska, M.J. and Poon, Z. and Pracht, K. and Prinz, I. and Pucillo, C.E.M. and Quataert, S.A. and Quatrini, L. and Quinn, K.M. and Radbruch, H. and Radstake, T.R.D.J. and Rahmig, S. and Rahn, H.P. and Rajwa, B. and Ravichandran, G. and Raz, Y. and Rebhahn, J.A. and Recktenwald, D. and Reimer, D. and Reis E Sousa, C. and Remmerswaal, E.B.M. and Richter, L. and Rico, L.G. and Riddell, A. and Rieger, A.M. and Robinson, J.P and Romagnani, C. and Rubartelli, A. and Ruland, J. and Saalmüller, A. and Saeys, Y. and Saito, T. and Sakaguchi, S. and Sala-de-Oyanguren, F. and Samstag, Y. and Sanderson, S. and Sandrock, I. and Santoni, A. and Sanz, R.B. and Saresella, M. and Sautes-Fridman, C. and Sawitzki, B. and Schadt, L. and Scheffold, A. and Scherer, H.U and Schiemann, M. and Schildberg, F.A. and Schimisky, E. and Schlitzer, A. and Schlosser, J. and Schmid, S. and Schmitt, S. and Schober, K. and Schraivogel, D. and Schuh, W. and Schüler, T. and Schulte, R. and Schulz, A.R. and Schulz, S.R. and Scottá, C. and Scott-Algara, D. and Sester, D.P. and Shankey, T.V. and Silva-Santos, B. and Simon, A.K. and Sitnik, K.M. and Sozzani, S. and Speiser, D.E. and Spidlen, J. and Stahlberg, A. and Stall, A.M. and Stanley, N. and Stark, R. and Stehle, C. and Steinmetz, T. and Stockinger, H. and Takahama, Y. and Takeda, K. and Tan, L. and Tárnok, A. and Tiegs, G. and Toldi, G. and Tornack, J. and Traggiai, E. and Trebak, M. and Tree, T.I.M. and Trotter, J. and Trowsdale, J. and Tsoumakidou, M. and Ulrich, H. and Urbanczyk, S. and van de Veen, W. and van den Broek, M. and van der Pol, E. and Van Gassen, S. and Van Isterdael, G. and van Lier, R.A.W. and Veldhoen, M. and Vento-Asturias, S. and Vieira, P. and Voehringer, D. and Volk, H.D. and von Borstel, A. and von Volkmann, K. and Waisman, A. and Walker, R.V. and Wallace, P.K. and Wang, S.A. and Wang, X.M. and Ward, M.D. and Ward-Hartstonge, K.A. and Warnatz, K. and Warnes, G. and Warth, S. and Waskow, C. and Watson, J.V. and Watzl, C. and Wegener, L. and Weisenburger, T. and Wiedemann, A. and Wienands, J. and Wilharm, A. and Wilkinson, R.J. and Willimsky, G. and Wing, J.B. and Winkelmann, R. and Winkler, T.H. and Wirz, O.F. and Wong, A. and Wurst, P. and Yang, J.H.M. and Yang, J. and Yazdanbakhsh, M. and Yu, L. and Yue, A. and Zhang, H. and Zhao, Y. and Ziegler, S.M. and Zielinski, C. and Zimmermann, J. and Zychlinsky, A.
Abstract:These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Keywords:Allergy and Immunology, Cell Separation, Consensus, Flow Cytometry, Phenotype
Source:European Journal of Immunology
Page Range:1457-1973
Date:October 2019
Official Publication:https://doi.org/10.1002/eji.201970107
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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