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Long and repeat-rich intronic sequences favor circular RNA formation under conditions of reduced spliceosome activity

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Item Type:Article
Title:Long and repeat-rich intronic sequences favor circular RNA formation under conditions of reduced spliceosome activity
Creators Name:Wang, M. and Hou, J. and Müller-McNicoll, M. and Chen, W. and Schuman, E.M.
Abstract:Circular RNAs (circRNAs), an important class of regulatory RNAs, have been shown to be the most prevalent in the brain compared with other tissues. However the processes governing their biogenesis in neurons are still elusive. Moreover, little is known about whether and how different biogenesis factors work in synchrony to generate neuronal circRNAs. To address this question, we pharmacologically inhibited the spliceosome and profiled rat neuronal circRNAs using RNA sequencing. We identified over 100 circRNAs that were up-regulated and a few circRNAs that were down-regulated upon spliceosome inhibition. Bioinformatic analysis revealed that up-regulated circRNAs possess significantly longer flanking introns compared with the un-changed circRNA population. Moreover, the flanking introns of up-regulated circRNAs harbor a higher number of distinct repeat sequences and more reverse complementary motifs compared with the unchanged circRNAs. Taken together, our data demonstrate that the biogenesis of circRNAs containing distinct intronic features becomes favored under conditions of limited spliceosome activity.
Source:iScience
ISSN:2589-0042
Publisher:Cell Press (U.S.A.)
Volume:20
Page Range:237-247
Date:25 October 2019
Official Publication:https://doi.org/10.1016/j.isci.2019.08.058
PubMed:View item in PubMed

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