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Genomic DNA transposition induced by human PGBD5

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Item Type:Article
Title:Genomic DNA transposition induced by human PGBD5
Creators Name:Henssen, A.G. and Henaff, E. and Jiang, E. and Eisenberg, A.R. and Carson, J.R. and Villasante, C.M. and Ray, M. and Still, E. and Burns, M. and Gandara, J. and Feschotte, C. and Mason, C.E. and Kentsis, A.
Abstract:Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containing inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic remodeling contributes to its biological function.
Keywords:DNA Transposable Elements, Genetic Recombination, Substrate Specificity, Transposases
Source:eLife
ISSN:2050-084X
Publisher:eLife Sciences Publications (U.K.)
Volume:4
Page Range:e10565
Date:25 September 2015
Official Publication:https://doi.org/10.7554/eLife.10565
PubMed:View item in PubMed
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https://edoc.mdc-berlin.de/18407/Preprint version

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