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Forward genetic screen of human transposase genomic rearrangements

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Item Type:Article
Title:Forward genetic screen of human transposase genomic rearrangements
Creators Name:Henssen, A.G. and Jiang, E. and Zhuang, J. and Pinello, L. and Socci, N.D. and Koche, R. and Gonen, M. and Villasante, C.M. and Armstrong, S.A. and Bauer, D.E. and Weng, Z. and Kentsis, A.
Abstract:BACKGROUND: Numerous human genes encode potentially active DNA transposases or recombinases, but our understanding of their functions remains limited due to shortage of methods to profile their activities on endogenous genomic substrates. RESULTS: To enable functional analysis of human transposase-derived genes, we combined forward chemical genetic hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) screening with massively parallel paired-end DNA sequencing and structural variant genome assembly and analysis. Here, we report the HPRT1 mutational spectrum induced by the human transposase PGBD5, including PGBD5-specific signal sequences (PSS) that serve as potential genomic rearrangement substrates. CONCLUSIONS: The discovered PSS motifs and high-throughput forward chemical genomic screening approach should prove useful for the elucidation of endogenous genome remodeling activities of PGBD5 and other domesticated human DNA transposases and recombinases.
Keywords:Inverted Terminal Repeat, Thioguanine, HPRT1 Gene, Recombination Signal Sequence, Short Tandem Repeat Analysis
Source:BMC Genomics
ISSN:1471-2164
Publisher:BioMed Central (U.K.)
Volume:17
Page Range:548
Date:4 August 2016
Official Publication:https://doi.org/10.1186/s12864-016-2877-x
PubMed:View item in PubMed
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https://edoc.mdc-berlin.de/18408/Preprint version

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