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Linkage evidence for a two-locus inheritance of LQT-associated seizures in a multigenerational LQT family with a novel KCNQ1 loss-of-function mutation

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Title:Linkage evidence for a two-locus inheritance of LQT-associated seizures in a multigenerational LQT family with a novel KCNQ1 loss-of-function mutation
Creators Name:Prüss, H. and Gessner, G. and Heinemann, S.H. and Rüschendorf, F. and Ruppert, A.K. and Schulz, H. and Sander, T. and Rimpau, W.
Abstract:Mutations in several genes encoding ion channels can cause the long-QT (LQT) syndrome with cardiac arrhythmias, syncope and sudden death. Recently, mutations in some of these genes were also identified to cause epileptic seizures in these patients, and the sudden unexplained death in epilepsy (SUDEP) was considered to be the pathologic overlap between the two clinical conditions. For LQT-associated KCNQ1 mutations, only few investigations reported the coincidence of cardiac dysfunction and epileptic seizures. Clinical, electrophysiological and genetic characterization of a large pedigree (n = 241 family members) with LQT syndrome caused by a 12-base-pair duplication in exon 8 of the KCNQ1 gene duplicating four amino acids in the carboxyterminal KCNQ1 domain (KCNQ1dup12; p.R360_Q361dupQKQR, NM_000218.2, hg19). Electrophysiological recordings revealed no substantial KCNQ1-like currents. The mutation did not exhibit a dominant negative effect on wild-type KCNQ1 channel function. Most likely, the mutant protein was not functionally expressed and thus not incorporated into a heteromeric channel tetramer. Many LQT family members suffered from syncopes or developed sudden death, often after physical activity. Of 26 family members with LQT, seizures were present in 14 (LQTplus seizure trait). Molecular genetic analyses confirmed a causative role of the novel KCNQ1dup12 mutation for the LQT trait and revealed a strong link also with the LQTplus seizure trait. Genome-wide parametric multipoint linkage analyses identified a second strong genetic modifier locus for the LQTplus seizure trait in the chromosomal region 10p14. The linkage results suggest a two-locus inheritance model for the LQTplus seizure trait in which both the KCNQ1dup12 mutation and the 10p14 risk haplotype are necessary for the occurrence of LQT-associated seizures. The data strongly support emerging concepts that KCNQ1 mutations may increase the risk of epilepsy, but additional genetic modifiers are necessary for the clinical manifestation of epileptic seizures.
Keywords:LQT Syndrome, KCNQ1, Familial Epilepsy, Risk Haplotype, Genetic Modifier
Source:Frontiers in Neurology
Publisher:Frontiers Media SA
Page Range:648
Date:June 2019
Official Publication:https://doi.org/10.3389/fneur.2019.00648
PubMed:View item in PubMed

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