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Scalable workflows and reproducible data analysis for genomics

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Item Type:Article
Title:Scalable workflows and reproducible data analysis for genomics
Creators Name:Strozzi, F. and Janssen, R. and Wurmus, R. and Crusoe, M.R. and Githinji, G. and Di Tommaso, P. and Belhachemi, D. and Möller, S. and Smant, G. and de Ligt, J. and Prins, P.
Abstract:Biological, clinical, and pharmacological research now often involves analyses of genomes, transcriptomes, proteomes, and interactomes, within and between individuals and across species. Due to large volumes, the analysis and integration of data generated by such high-throughput technologies have become computationally intensive, and analysis can no longer happen on a typical desktop computer.In this chapter we show how to describe and execute the same analysis using a number of workflow systems and how these follow different approaches to tackle execution and reproducibility issues. We show how any researcher can create a reusable and reproducible bioinformatics pipeline that can be deployed and run anywhere. We show how to create a scalable, reusable, and shareable workflow using four different workflow engines: the Common Workflow Language (CWL), Guix Workflow Language (GWL), Snakemake, and Nextflow. Each of which can be run in parallel.We show how to bundle a number of tools used in evolutionary biology by using Debian, GNU Guix, and Bioconda software distributions, along with the use of container systems, such as Docker, GNU Guix, and Singularity. Together these distributions represent the overall majority of software packages relevant for biology, including PAML, Muscle, MAFFT, MrBayes, and BLAST. By bundling software in lightweight containers, they can be deployed on a desktop, in the cloud, and, increasingly, on compute clusters.By bundling software through these public software distributions, and by creating reproducible and shareable pipelines using these workflow engines, not only do bioinformaticians have to spend less time reinventing the wheel but also do we get closer to the ideal of making science reproducible. The examples in this chapter allow a quick comparison of different solutions.
Keywords:Bioinformatics, Evolutionary Biology, Big Data, Parallelization, MPI, Cloud Computing, Cluster Computing, Virtual Machine, MrBayes, Debian Linux, GNU Guix, Bioconda, CWL, Common Workflow Language, Guix Workflow Language, Snakemake, Nextflow
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Evolutionary Genomics
ISSN:1064-3745
ISBN:978-1-4939-9073-3
Publisher:Springer / Humana Press (U.S.A.)
Volume:1910
Page Range:723-745
Date:2019
Official Publication:https://doi.org/10.1007/978-1-4939-9074-0_24
PubMed:View item in PubMed

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