Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers

Item Type:	Conference or Workshop Item
Title:	Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers
Creators Name:	Kemper, B. and Pohl, L. and Kaiser, M. and Doepker, E. and Schnekenburger, J. and Ketelhut, S.
Abstract:	We have explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images for example of digital holographic microscopy (DHM), a variant of quantitative phase microscopy (QPM). The applicability of the proposed numerical procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. The achieved results show that histogram-based evaluation of quantitative phase images allows a highly reliable detection and continuous observation of global cellular morphology changes in confluent cell layers.
Keywords:	Quantitative Phase Imaging, Digital Holographic Microscopy, Label-Free Live Cell Imaging, Confluent Cell Layers
Source:	Proceedings of SPIE
Series Name:	Proceedings of SPIE
Title of Book:	Quantitative Phase Imaging V
ISSN:	0277-786X
Publisher:	International Society for Optics and Photonics (SPIE)
Volume:	10887
Page Range:	108871M-1
Date:	4 March 2019
Official Publication:	https://doi.org/10.1117/12.2509242

Repository Staff Only: item control page