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Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE/AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland

Item Type:Article
Title:Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE/AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland
Creators Name:Haendler, B. and Kraetzschmar, J. and Theuring, F. and Schleuning, W.D.
Abstract:Expression of the gene for cysteine-rich secretory protein-1 (CRISP-1), the counterpart of the rat sperm-coating protein DE/AEG, was evidenced in male mouse salivary gland by both isolation of the corresponding cDNA clone and RNA blot analysis. In addition, the cDNA coding for the new related CRISP-3, which shares 77% amino acid identity with CRISP-1, was isolated from a salivary gland cDNA library. The activity of CRISP-1 and -3 genes in mouse salivary gland is strongly androgen dependent, as seen in castrated males; also, no CRISP-1 and only weak CRISP-3 expression was detected in female salivary gland. A variety of tissues were tested for the presence of CRISP-3 transcripts, but specific signals were only found in salivary gland. The results of genomic DNA blot analysis are compatible with the existence of single discrete genes for CRISP-1 and CRISP-3.
Keywords:Amino Acid Sequence, Androgens, Base Sequence, Northern Blotting, Southern Blotting, Molecular Cloning, DNA, Gene Expression, Membrane Glycoproteins, Molecular Sequence Data, Messenger RNA, Salivary Glands, Salivary Proteins and Peptides, Seminal Plasma Proteins, Sequence Homology, Tissue Distribution, Genetic Transcription, Animals, Mice
Source:Endocrinology
ISSN:0013-7227
Publisher:Endocrine Society (U.S.A.)
Volume:133
Number:1
Page Range:192-198
Date:1 July 1993
Official Publication:http://endo.endojournals.org/cgi/content/abstract/133/1/192
PubMed:View item in PubMed

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