Early hearing loss upon disruption of Slc4a10 in C57BL/6 mice

Item Type:	Article
Title:	Early hearing loss upon disruption of Slc4a10 in C57BL/6 mice
Creators Name:	Huebner, A.K. and Maier, H. and Maul, A. and Nietzsche, S. and Herrmann, T. and Praetorius, J. and Hübner, C.A.
Abstract:	The unique composition of the endolymph with a high extracellular K(+) concentration is essential for sensory transduction in the inner ear. It is secreted by a specialized epithelium, the stria vascularis, that is connected to the fibrocyte meshwork of the spiral ligament in the lateral wall of the cochlea via gap junctions. In this study, we show that in mice the expression of the bicarbonate transporter Slc4a10/Ncbe/Nbcn2 in spiral ligament fibrocytes starts shortly before hearing onset. Its disruption in a C57BL/6 background results in early onset progressive hearing loss. This hearing loss is characterized by a reduced endocochlear potential from hearing onset onward and progressive degeneration of outer hair cells. Notably, the expression of a related bicarbonate transporter, i.e., Slc4a7/Nbcn1, is also lost in spiral ligament fibrocytes of Slc4a10 knockout mice. The histological analysis of the spiral ligament of Slc4a10 knockout mice does not reveal overt fibrocyte loss as reported for Slc4a7 knockout mice. The ultrastructural analysis, however, shows mitochondrial alterations in fibrocytes of Slc4a10 knockout mice. Our data suggest that Slc4a10 and Slc4a7 are functionally related and essential for inner ear homeostasis.
Keywords:	Bicarbonate Transport, Slc4a10, Slc4a7, NCBE, Deafness, Fibrocyte, pH, Animals, Mice
Source:	Journal of the Association for Research in Otolaryngology
ISSN:	1525-3961
Publisher:	Springer
Volume:	20
Number:	3
Page Range:	233-245
Date:	June 2019
Additional Information:	This article will also be published in open access. It has a delayed release (embargo) and will be available in PMC on June 1, 2020.
Official Publication:	https://doi.org/10.1007/s10162-019-00719-1
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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