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Fluconazole increases osmotic water transport in renal collecting duct through effects on aquaporin-2 trafficking

Item Type:Article
Title:Fluconazole increases osmotic water transport in renal collecting duct through effects on aquaporin-2 trafficking
Creators Name:Vukićević, T. and Hinze, C. and Baltzer, S. and Himmerkus, N. and Quintanova, C. and Zühlke, K. and Compton, F. and Ahlborn, R. and Dema, A. and Eichhorst, J. and Wiesner, B. and Bleich, M. and Schmidt-Ott, K.M. and Klussmann, E.
Abstract:BACKGROUND: Arginine-vasopressin (AVP) binding to vasopressin V2 receptors promotes redistribution of the water channel aquaporin-2 (AQP2) from intracellular vesicles into the plasma membrane of renal collecting duct principal cells. This pathway fine-tunes renal water reabsorption and urinary concentration, and its perturbation is associated with diabetes insipidus. Previously, we identified the antimycotic drug fluconazole as a potential modulator of AQP2 localization. METHODS: We assessed the influence of fluconazole on AQP2 localization in vitro and in vivo as well as the drug's effects on AQP2 phosphorylation and RhoA (a small GTPase, which under resting conditions, maintains F-actin to block AQP2-bearing vesicles from reaching the plasma membrane). We also tested fluconazole's effects on water flow across epithelia of isolated mouse collecting ducts and on urine output in mice treated with tolvaptan, a VR2 blocker that causes a nephrogenic diabetes insipidus-like excessive loss of hypotonic urine. RESULTS: Fluconazole increased plasma membrane localization of AQP2 in principal cells independent of AVP. It also led to an increased AQP2 abundance associated with alterations in phosphorylation status and ubiquitination as well as inhibition of RhoA. In isolated mouse collecting ducts, fluconazole increased transepithelial water reabsorption. In mice, fluconazole increased collecting duct AQP2 plasma membrane localization and reduced urinary output. Fluconazole also reduced urinary output in tolvaptan-treated mice. CONCLUSIONS: Fluconazole promotes collecting duct AQP2 plasma membrane localization in the absence of AVP. Therefore, it might have utility in treating forms of diabetes insipidus (e.g., X-linked nephrogenic diabetes insipidus) in which the kidney responds inappropriately to AVP.
Keywords:Cyclic AMP, Diabetes Insipidus, Intracellular Signal, Renal Cell Biology, Vasopressin, Water Channels, Animals, Mice
Source:Journal of the American Society of Nephrology
Publisher:American Society of Nephrology
Page Range:795-810
Date:May 2019
Official Publication:https://doi.org/10.1681/ASN.2018060668
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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