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Characterization of mouse adult testicular macrophage populations by immunofluorescence imaging and flow cytometry

Item Type:Article
Title:Characterization of mouse adult testicular macrophage populations by immunofluorescence imaging and flow cytometry
Creators Name:Mossadegh-Keller, N. and Sieweke, M.H.
Abstract:Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive cytokines and trophic roles in supporting spermatogenesis and male sex hormone production. They also contribute to fetal testicular development. Recently, we characterized two distinct tMΦ populations based on their morphology, localization, cell surface markers, and gene expression profiling. Here, we focus and describe in detail the phenotypical distinction of these two tMΦ populations by fluorescence-activated cell sorting (FACS) using multicolor panel antibodies combining with high-resolution immunofluorescence (IF) imaging. These two techniques enable to classify two tMΦ populations: interstitial tMΦ and peritubular tMΦ.
Keywords:Macrophage, Testis, FACS, IF, Spermatogenesis, Immune-Privilege Organ, Animals, Mice
Source:Bio-protocol
ISSN:2331-8325
Publisher:Bio-protocol LLC (U.S.A.)
Volume:9
Number:5
Page Range:e3178
Date:5 March 2019
Official Publication:https://doi.org/10.21769/BioProtoc.3178
PubMed:View item in PubMed

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