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A gene expression atlas of embryonic neurogenesis in Drosophila reveals complex spatiotemporal regulation of lncRNAs

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Item Type:Article
Title:A gene expression atlas of embryonic neurogenesis in Drosophila reveals complex spatiotemporal regulation of lncRNAs
Creators Name:McCorkindale, A.L. and Wahle, P. and Werner, S. and Jungreis, I. and Menzel, P. and Shukla, C.J. and Abreu, R.L.P. and Irizarry, R.A. and Meyer, I.M. and Kellis, M. and Zinzen, R.P.
Abstract:Cell type specification during early nervous system development in Drosophila melanogaster requires precise regulation of gene expression in time and space. Resolving the programs driving neurogenesis has been a major challenge owing to the complexity and rapidity with which distinct cell populations arise. To resolve the cell type-specific gene expression dynamics in early nervous system development, we have sequenced the transcriptomes of purified neurogenic cell types across consecutive time points covering crucial events in neurogenesis. The resulting gene expression atlas comprises a detailed resource of global transcriptome dynamics that permits systematic analysis of how cells in the nervous system acquire distinct fates. We resolve known gene expression dynamics and uncover novel expression signatures for hundreds of genes among diverse neurogenic cell types, most of which remain unstudied. We also identified a set of conserved long noncoding RNAs (lncRNAs) that are regulated in a tissue-specific manner and exhibit spatiotemporal expression during neurogenesis with exquisite specificity. lncRNA expression is highly dynamic and demarcates specific subpopulations within neurogenic cell types. Our spatiotemporal transcriptome atlas provides a comprehensive resource for investigating the function of coding genes and noncoding RNAs during crucial stages of early neurogenesis.
Keywords:Embryogenesis, lncRNA, Spatiotemporal Transcriptome, Neurogenesis, Animals, Drosophila melanogaster
Source:Development
ISSN:0950-1991
Publisher:Company of Biologists (U.K.)
Volume:146
Number:6
Page Range:dev175265
Date:28 March 2019
Official Publication:https://doi.org/10.1242/dev.175265
PubMed:View item in PubMed
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https://edoc.mdc-berlin.de/17967/Final version

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