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Live-imaging readouts and cell models for phenotypic profiling of mitochondrial function

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Item Type:Review
Title:Live-imaging readouts and cell models for phenotypic profiling of mitochondrial function
Creators Name:Iannetti, E.F. and Prigione, A. and Smeitink, J.A.M. and Koopman, W.J.H. and Beyrath, J. and Renkema, H.
Abstract:Mitochondria are best known as the powerhouses of the cells but their cellular role goes far beyond energy production; among others, they have a pivotal function in cellular calcium and redox homeostasis. Mitochondrial dysfunction is often associated with severe and relatively rare disorders with an unmet therapeutic need. Given their central integrating role in multiple cellular pathways, mitochondrial dysfunction is also relevant in the pathogenesis of various other, more common, human pathologies. Here we discuss how live-cell high content microscopy can be used for image-based phenotypic profiling to assess mitochondrial (dys) function. From this perspective, we discuss a selection of live-cell fluorescent reporters and imaging strategies and discuss the pros/cons of human cell models in mitochondrial research. We also present an overview of live-cell high content microscopy applications used to detect disease-associated cellular phenotypes and perform cell-based drug screening.
Keywords:Mitochondrial Disease, Pathological Phenotype, Cell Models Of Disease, Assay Development, Live Cell Microscopy, Fluorescent Probes, HCS, Cellomics
Source:Frontiers in Genetics
Publisher:Frontiers Media SA
Page Range:131
Date:March 2019
Official Publication:https://doi.org/10.3389/fgene.2019.00131
PubMed:View item in PubMed

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