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Dendritic cell progenitor is transformed by a conditional v-rel estrogen receptor fusion v-relER

Item Type:Article
Title:Dendritic cell progenitor is transformed by a conditional v-rel estrogen receptor fusion v-relER
Creators Name:Boehmelt, G. and Madruga, J. and Doerfler, P. and Briegel, K. and Schwarz, H. and Enrietto, P. and Zenke, M.
Abstract:A conditional v-Rel estrogen receptor fusion protein, v-RelER, causes estrogen-dependent but otherwise unaltered v-rel-specific transformation of chicken bone marrow cells. Here, we demonstrate that such v-relER-transformed cells exhibit B lymphoid determinants in line with earlier studies on v-rel-transformed cells. However, following inactivation of v-RelER oncoprotein activity by administration of an estrogen antagonist, cells differentiate into antigen-presenting dendritic cells as judged by several morphological and functional criteria. Additionally, under yet different culture conditions, v-relER cells differentiate into cells resembling polymorphonuclear neutrophils. Our studies therefore suggest that the conditional v-RelER, and probably also the authentic v-Rel, transform a common progenitor for neutrophils and dendritic cells.
Keywords:B-Lymphocytes, Bone Marrow, Cell Differentiation, Transformed Cell Line, Dendritic Cells, Dendritic Cells, Fibroblasts, Flow Cytometry, Hematopoietic Stem Cells, Mixed Lymphocyte Culture Test, Macrophages, Electron Microscopy, Neutrophils, Oncogene Proteins v-rel, Oncogenes, Phagocytosis, Estrogen Receptors, Recombinant Fusion Proteins, Oncogenic Retroviridae Proteins, T-Lymphocytes, Transcription Factors, Vimentin, Animals, Chick Embryo
Source:Cell
ISSN:0092-8674
Publisher:Cell Press (U.S.A.)
Volume:80
Number:2
Page Range:341-352
Date:27 January 1995
Official Publication:https://doi.org/10.1016/0092-8674(95)90417-4
PubMed:View item in PubMed

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