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The essence of DNA sample preparation for MALDI mass spectrometry

Item Type:Review
Title:The essence of DNA sample preparation for MALDI mass spectrometry
Creators Name:Sauer, S.
Abstract:Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has become an important analytical technique in nucleic acid research. MALDI is used for quality control of oligonucleotides as well as for analyzing DNA markers. Sample preparation of nucleic acids is crucial for obtaining high-quality mass spectra. Sample purity, solvent content, suitable matrices, and substrate surfaces, as well as laboratory conditions affect spectra quality. This review presents essential information with regard to sample preparation, DNA modification chemistry, and DNA purification, along with a discussion of instrumental advances, which facilitate and extend the applicability of MALDI in genomics.
Keywords:MALDI, DNA, Oligonucleotide, Matrix, Purification, SNP
Source:Journal of Biochemical and Biophysical Methods
ISSN:0165-022X
Publisher:Biophysical Society (U.S.A.)
Volume:70
Number:2
Page Range:311-318
Date:10 March 2007
Official Publication:https://doi.org/10.1016/j.jbbm.2006.10.007
PubMed:View item in PubMed

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