Automated solid-phase extraction for purification of single nucleotide polymorphism genotyping products prior to matrix-assisted laser desorption/ionisation time-of-flight mass spectrometric analysis

Item Type:	Article
Title:	Automated solid-phase extraction for purification of single nucleotide polymorphism genotyping products prior to matrix-assisted laser desorption/ionisation time-of-flight mass spectrometric analysis
Creators Name:	Sauer, S. and Kepper, P. and Smyra, A. and Dahl, A. and Ferse, F.T. and Lehrach, H. and Reinhardt, R.
Abstract:	In this article, we describe the application of a novel micro elution solid-phase extraction method for purification of short stretches of DNA products of single nucleotide polymorphisms (SNPs) prior to MALDI mass spectrometry analysis. An important feature of our method is that the purification columns containing a copolymer of divinylbenzene and N-vinylpyrrolidone can be used several times thereby significantly reducing costs. We implemented this DNA purification technology into a fully automated procedure including molecular biology, MALDI sample preparation, automated mass spectrometric analysis and SNP allele calling by software. Due to the facile purification protocol, the methodology shown could furthermore be used for other applications where efficient medium to high-throughput purification of nucleic acids is required.
Keywords:	Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry, Single Nucleotide Polymorphism, Solid-Phase Extraction, Genotyping, Purification
Source:	Journal of Chromatography A
ISSN:	0021-9673
Publisher:	Elsevier
Volume:	1049
Number:	1-2
Page Range:	9-16
Date:	17 September 2004
Official Publication:	https://doi.org/10.1016/j.chroma.2004.07.069
PubMed:	View item in PubMed

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