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BCR-dependent lineage plasticity in mature B cells

Item Type:Article
Title:BCR-dependent lineage plasticity in mature B cells
Creators Name:Graf, R. and Seagal, J. and Otipoby, K.L. and Lam, K.P. and Ayoub, S. and Zhang, B. and Sander, S. and Chu, V.T. and Rajewsky, K.
Abstract:B2 cells engage in classical antibody responses, whereas B1 cells are considered carriers of innate immunity, biased toward recognizing epitopes present on the surfaces of common pathogens and self antigens. To explore the role of B cell antigen receptor (BCR) specificity in driving B1 cell differentiation, we developed a transgenic system allowing us to change BCR specificity in B cells in an inducible and programmed manner. Mature B2 cells differentiated into bona fide B1 cells upon acquisition of a B1 cell-typical self-reactive BCR through a phase of proliferative expansion. Thus, B2 cells have B1 cell differentiation potential in addition to their classical capacity to differentiate into memory and plasma cells, and B1 differentiation can be instructed by BCR-mediated self-reactivity, in the absence of B1-lineage precommitment.
Keywords:B-Cell Antigen Receptors, B-Lymphocyte Subsets, Cell Differentiation, Cell Lineage, Cell Plasticity, Immunoglobulin Class Switching, Immunoglobulin Heavy Chains, Immunoglobulin Variable Region, Transcriptome, Transgenic Mice, Animals, Mice
Publisher:American Association for the Advancement of Science
Page Range:748-753
Date:15 February 2019
Official Publication:https://doi.org/10.1126/science.aau8475
PubMed:View item in PubMed

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