Helmholtz Gemeinschaft


RNA tomography for spatially resolved transcriptomics (Tomo-seq)

Item Type:Article
Title:RNA tomography for spatially resolved transcriptomics (Tomo-seq)
Creators Name:Holler, K. and Junker, J.P.
Abstract:Embryonic development is heavily dependent on temporally and spatially restricted gene expression. Spatially resolved measurements of gene expression are therefore crucial for identifying novel regulators and the understanding of their function. However, in situ methods do not resolve global gene expression, and sequencing-based methods usually do not provide spatial information. Here, we describe tomo-seq, a method that combines classical histological sectioning of embryos or tissues with a highly sensitive RNA-sequencing technique. Application of tomo-seq to zebrafish embryos allows reconstructing the spatial gene expression of thousands of genes.
Keywords:mRNA Sequencing, Local Gene Expression, Spatial Transcriptomics, Developmental Patterning, Gene Expression Mapping, Morphogen Gradients, Animals, Zebrafish
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Vertebrate embryogenesis : embryological, cellular, and genetic methods
Publisher:Springer / Humana Press
Page Range:129-141
Official Publication:https://doi.org/10.1007/978-1-4939-9009-2_9
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library