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Autocrine LTA signaling drives NF-κB and JAK-STAT activity and myeloid gene expression in Hodgkin lymphoma

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Item Type:Article
Title:Autocrine LTA signaling drives NF-κB and JAK-STAT activity and myeloid gene expression in Hodgkin lymphoma
Creators Name:von Hoff, L. and Kärgel, E. and Franke, V. and McShane, E. and Schulz-Beiss, K.W. and Patone, G. and Schleussner, N. and Kolesnichenko, M. and Hübner, N. and Daumke, O. and Selbach, M. and Akalin, A. and Mathas, S. and Scheidereit, C.
Abstract:Persistent NF-κB activation is a hallmark of the malignant Hodgkin/Reed-Sternberg (HRS) cells in classical Hodgkin lymphoma (cHL). Genomic lesions, Epstein-Barr virus infection, soluble factors and tumor-microenvironment interactions contribute to this activation. Here, in an unbiased approach to identify the cHL cell-secreted key factors for NF-κB activation, we have dissected the secretome of cultured cHL cells by chromatography and subsequent mass spectrometry. We identified lymphotoxin-α (LTA) as the causative factor for autocrine and paracrine activation of canonical and non-canonical NF-κB in cHL cell lines. Apart from inducing NF-κB, LTA promotes JAK2/STAT6 signaling. LTA and its receptor TNFRSF14 are transcriptionally activated by non-canonical NF-κB, creating a continuous feedback loop. Furthermore, LTA shapes the expression of cytokines, receptors, immune checkpoint ligands and adhesion molecules, including CSF2, CD40, PD-L1/-L2 and VCAM1. Comparison with single cell gene-activity profiles of human hematopoietic cells showed that LTA not only induces genes restricted to the lymphoid but also largely to the myeloid lineage. Thus, LTA sustains autocrine NF-κB activation, impacts activation of several signaling pathways and drives expression of genes essential for microenvironmental interactions and lineage ambiguity. These data provide a robust rationale for LTA-targeting as a treatment strategy for cHL patients.
Keywords:Cell Line, Hodgkin Disease, Janus Kinase 2, Lymphotoxin-alpha, NF-kappa B, Neoplastic Gene Expression Regulation, Reed-Sternberg Cells, STAT6 Transcription Factor, Signal Transduction, Transcriptional Activation
Publisher:American Society of Hematology
Page Range:1489-1494
Date:28 March 2019
Additional Information:Copyright © 2019 by The American Society of Hematology
Official Publication:https://doi.org/10.1182/blood-2018-08-871293
PubMed:View item in PubMed

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