Collagen I-based scaffolds negatively impact fracture healing in a mouse-osteotomy-model although used routinely in research and clinical application

Item Type:	Article
Title:	Collagen I-based scaffolds negatively impact fracture healing in a mouse-osteotomy-model although used routinely in research and clinical application
Creators Name:	Lang, A. and Kirchner, M. and Stefanowski, J. and Durst, M. and Weber, M.C. and Pfeiffenberger, M. and Damerau, A. and Hauser, A.E. and Hoff, P. and Duda, G.N. and Buttgereit, F. and Schmidt-Bleek, K. and Gaber, T.
Abstract:	Although several biomaterials for bone regeneration have been developed in the last decades, clinical application of bone morphogenetic protein 2 is clinically only approved when applied on an absorbable bovine collagen I scaffold (ACS) (Helistat; ACS-H). In research, another ACS, namely Lyostypt (ACS-L) is frequently used as a scaffold in bone-linked studies. Nevertheless, until today, the influence of ACS alone on bone healing remains unknown. Unexpectedly, in vitro studies using ASC-H revealed a suppression of osteogenic differentiation and a significant reduction of cell vitality when compared to ASC-L. In mice, we observed a significant delay in bone healing when applying ACS-L in the fracture gap during femoral osteotomy. The results of our study show for the first time a negative influence of both ACS-H and ACS-L on bone formation demonstrating a substantial need for more sophisticated delivery systems for local stimulation of bone healing in both clinical application and research.
Keywords:	Absorbable Bovine Collagen I Scaffold, Delayed Bone Healing, Non-Collagenous Proteins, Osteotomy Model, Animals, Cattle, Mice
Source:	Acta Biomaterialia
ISSN:	1742-7061
Publisher:	Elsevier
Volume:	86
Page Range:	171-184
Date:	1 March 2019
Official Publication:	https://doi.org/10.1016/j.actbio.2018.12.043
PubMed:	View item in PubMed

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