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Increased serum levels of granulocyte colony-stimulating factor after autologous bone marrow or blood stem cell transplantation

Item Type:Article
Title:Increased serum levels of granulocyte colony-stimulating factor after autologous bone marrow or blood stem cell transplantation
Creators Name:Haas, R. and Gericke, G. and Witt, B. and Cayeux, S. and Hunstein, W.
Abstract:The objective of our study was to evaluate the biologic role of granulocyte colony-stimulating factor (G-CSF) for hematologic reconstitution following autologous bone marrow transplantation (ABSCT). Using a commercially available enzyme-linked immunosorbent assay, serum levels of G-CSF were measured in samples from 48 patients (30 male/18 female) who underwent ABMT or ABSCT. Their median age was 34.5 years (range 16 to 51). Autografting was performed (40 ABMT, 8 ABSCT) in 23 AML, 8 ALL and 17 malignant lymphoma patients. Patients transplanted with blood stem cells had a faster leukocyte and neutrophil recovery compared with the ABMT patients (p < 0.025 and p < 0.05, respectively). During marrow aplasia G-CSF serum levels were elevated in all patients, with a median peak value of 2199 pg/mL (range 453 to 8676 pg/mL). A strong reverse correlation (R = -0.76, p < 0.01) could be demonstrated between G-CSF serum level and white blood count (WBC). An additional increase of G-CSF serum levels on days of fever (> or = 38.5 degrees C) or documented infectious disease was observed. During the early phase of marrow aplasia, the endogenously produced amounts of G-CSF reached concentrations which are used for in vitro stimulation of colony-forming unit granulocyte (CFU-G). The relationship between G-CSF serum level and WBC supports the central role of this circulating hemopoietin following myeloablative treatment and autotransplantation. During periods of higher demand such as fever and infectious complications, endogenous G-CSF production is enhanced.
Keywords:Bone Marrow Transplantation, Enzyme-Linked Immunosorbent Assay, Granulocyte Colony-Stimulating Factor, Hematopoietic Stem Cell Transplantation, Infection, Myeloid, Acute Leukemia, Leukocyte Count, Leukocytes, Lymphoma, Neutrophils, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Autologous Transplantation
Source:Experimental Hematology
Page Range:109-113
Date:1 January 1993
PubMed:View item in PubMed

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